RNA

Part:BBa_K4811008:Design

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-09-25)


TMS(Neo1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RNA has to fold as a tRNA would.


Source

The part was developed by Avishek Paul during their PHD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

References

Jiang L, Majumdar A, Hu W, Jaishree TJ, Xu W, Patel DJ. Saccharide-RNA recognition in a complex formed between neomycin B and an RNA aptamer. Structure. 1999 Jul 15;7(7):817-27. doi: 10.1016/s0969-2126(99)80105-1. PMID: 10425683.

"Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.